Next-Generation Sequencing

Designed for individuals preparing genomic DNA libraries for sequencing on the SOLiD™ System or the 5500 Series Genetic Analyzer platform and seeking detailed knowledge of the molecular biology techniques used for SOLiD™ System library construction.

Ownership of a SOLiD™ System or 5500 Series Genetic Analyzer (or belonging to a laboratory that has purchased a system) is a prerequisite for registration. Ideally, individuals have already participated in the SOLiD™ System Training Course, although this is not mandatory. This class assumes attendees will have prior knowledge of biological systems and basic experience with laboratory techniques, such as pipetting and PCR.

A 5-day course involving classroom and laboratory sections for fragment and mate-paired library construction. Course includes in-depth discussions of SOLiD™ System library construction and the molecular biology strategies that are used in the workflow. (Please note, we will NOT be able to use customer samples for library construction during this course.) Each participant will have the option to choose either a low-input or high-input fragment library protocol. Upon completion of the fragment protocol, each participant will then construct a mate-paired library to be used for sequencing on the SOLiD™ System or 5500 Series Genetic Analyzer. Information and strategies for different sequencing applications and how these relate to library construction and preparation of genomic samples will also be provided.

Tuition includes laboratory reagents, consumables, course materials, breakfast, and lunch. Other incidental expenses relating to the training are the responsibility of the attendee.

Course Agenda: To view the course agenda, click here: SOLiD™ Library Construction Agenda

For information on our Foster City course schedule, please contact us at 650-554-3360 or email us at customertraining@lifetech.com

The course is designed for individuals who will be directly involved in generating libraries from RNA to run on a SOLiD™ System. Prerequisites: Ownership of a SOLiD™ System (or affiliation with a laboratory that has purchased a system) and prior knowledge and basic experience of molecular biology and laboratory techniques including polymerase chain reaction (PCR) and pipetting. Past attendance of the SOLiD&trade System Training Course is recommended.

This is a 3-day intensive course that gives participants a strong practical and theoretical knowledge of how to build SAGE libraries using the SOLiD™ Total RNA-Seq Kit, with tips & tricks to QC and troubleshoot libraries. (Please note, we will NOT be able to use customer samples for library construction during this course.)

Course Agenda: To view the course agenda, click here: SOLiD RNA Libraries Construction for SAGE Studies

Tuition includes hotel, laboratory reagents, consumables, course materials, breakfast, and lunch. Travel and other incidental expenses relating to the training are the responsibility of the attendee.

For information on our Foster City course schedule, please contact us at 650-554-3360 or email us at customertraining@lifetech.com

The course is designed for individuals who will be directly involved in generating libraries from RNA to run on a SOLiD™ System.

Ownership of a SOLiD™ System (or affiliation with a laboratory that has purchased a system) and prior knowledge and basic experience of molecular biology and laboratory techniques including polymerase chain reaction (PCR) and pipetting. Past attendance of the SOLiD™ System Training Course is recommended. Course Description:

This is a 3-day intensive course that gives participants a strong practical and theoretical knowledge of how to build whole transcriptome and small RNA libraries using the SOLiD™ Total RNA-Seq Kit, with tips & tricks to QC and troubleshoot libraries. (Please note, we will NOT be able to use customer samples for library construction during this course.)

Course Agenda: To view the course agenda, click here: SOLiD RNA Libraries Cnnstruction for Whole Transcriptome and Small RNAs Studies Agenda

Tuition includes hotel, laboratory reagents, consumables, course materials, breakfast, and lunch. Travel and other incidental expenses relating to the training are the responsibility of the attendee.

For information on our Foster City course schedule, please contact us at 650-554-3360 or email us at customertraining@lifetech.com

Designed for individuals generating targeted resequencing libraries with the Agilent SureSelect®** Target Enrichment System to run on a SOLiD&trade System.

Ownership of a SOLiD™ System (or belonging to a laboratory that has purchased a system) is a prerequisite for registration. Ideally, individuals have already participated in the SOLiD™ System Training Course, although this is not mandatory. This class assumes attendees will have prior knowledge of biological systems and basic experience with laboratory techniques, such as pipetting and PCR.

An approximately 3-day intensive course providing strong practical and theoretical knowledge of construction of targeted resequencing libraries. Participants will perform targeted enrichment of selected regions of the genome enrichment (with the Agilent SureSelect®** Target Enrichment System) and construct the resulting enriched library using genomic DNA provided by the training lab. (Please note, we will NOT be able to use customer samples for library construction during this course.) Theory behind enrichment and library construction and an in-depth evaluation of each critical stage of the workflow is reviewed. Participants will also be provided with best practices on library QC methods, laboratory preparation, and equipment calibration before embarking on the creation of their own libraries.

Tuition includes hotel, laboratory reagents, consumables, course materials, breakfast, and lunch. Travel and other incidental expenses relating to the training are the responsibility of the attendee.

**SureSelect® is a trademark of Agilent Technologies, Inc.

For information on our Foster City course schedule, please contact us at 650-554-3360 or email us at customertraining@lifetech.com

Designed for individuals preparing Methylation or ChIP-Seq libraries to run on the SOLiD™ System.

Ownership of a SOLiD™ System (or belonging to a laboratory that has purchased a system) is a prerequisite for registration. Ideally, individuals have already participated in the SOLiD&trade System Training Course, although this is not mandatory. This class assumes attendees will have prior knowledge of biological systems and basic experience with laboratory techniques, such as pipetting and PCR.

A 5-day intensive course providing a strong practical and theoretical knowledge of construction of Methylation enriched libraries and ChIP-Seq libraries using the MethylMiner™ and SOLiD™ ChIP-Seq Kits available through Life Technologies. (Please note, we will NOT be able to use customer samples for library construction during this course.)

Tuition includes hotel, laboratory reagents, consumables, course materials, breakfast, and lunch. Travel and other incidental expenses relating to the training are the responsibility of the attendee.

For information on our Foster City course schedule, please contact us at 650-554-3360 or email us at customertraining@lifetech.com

Course attendees should have an extensive background in bioinformatics, basic to advanced knowledge and navigation of Linux, and own a 5500 Series Genetic Analyzer (or have an affiliation with a laboratory that has purchased a system).

A 2-day course involving classroom presentations and hands-on analysis pipeline execution with sample data sets. This course is designed to introduce the bioinformatics tools available for the 5500 Series Genetic Analyzer to individuals who have a substantial background in bioinformatics (including data mining). The goal of this training is to enable successful utilization of the LifeScope™ Genomic Analysis Software with a focus on Mapping, Re-Sequencing, Whole Transcriptome and smRNA Analysis workflows. Hands-on exercises are performed using both Linux command-line and graphical user interfaces.

Tuition includes course materials, breakfast, and lunch. Travel, accommodation, and other incidental expenses relating to the training are the responsibility of the attendee.

Course Agenda: To view the course agenda, click here: Advanced SOLiD Data Analysis Training Agenda

To enroll for one of these sessions, or for general questions, please call: (800) 874-9868 ext 3360 or (650)554-3360 or email the SOLiD™ Training Coordinator.

For information on our Foster City course schedule, please contact us at 650-554-3360 or email us at customertraining@lifetech.com

Intended for first-time users of the SOLiD™ 4 System, or novice users wishing to have additional or refresher training.

A 5-day intensive overview of the Applied Biosystems next-generation sequencing technology, the SOLiD™ 4 System. Course topics include an overview of instrument operation and hardware, library preparation, emulsion PCR, bead preparation, bead deposition, quality control runs, troubleshooting, and analysis software. An overview of the chemistry used in the SOLiD™ 4 System will be provided, which is critical for the understanding the system and troubleshooting. Hands-on training includes initiating and monitoring several runs during class, creating beads for the first run of their system, setting up multiple emulsions, and performing a library titration to optimize emulsion PCR.

Tuition at customer site includes course materials. Available by request. Please call 650-554-3360 or email customertraining@lifetech.comfor more information or to register.

Intended for first-time users of the 5500 Series SOLiD™ Sequencers, or novice users wishing to have additional or refresher training.

A 5-day intensive overview of the Applied Biosystems next-generation sequencing technology, the 5500 Series SOLiD™ Sequencers. Course topics include an overview of instrument operation and hardware, library preparation, emulsion PCR, bead preparation, bead deposition, quality control runs, troubleshooting, and analysis software. An overview of the chemistry used in the 5500 SOLiD™ System will be provided, which is critical for the understanding the system and troubleshooting. Hands-on training includes initiating and monitoring several runs during class, creating beads for the first run of their system, setting up multiple emulsions, and performing a library titration to optimize emulsion PCR.

Tuition at customer site includes course materials. Available by request. Please call 650-554-3360 or email customertraining@lifetech.com for more information or to register.

This is a 1-day training provided in your laboratory. It is designed for either first-time users or laboratories who wish to train new laboratory personnel. Laboratory personnel will benefit by having training on their instrument in their working environment and by having time to ask questions and interact with Applied Biosystems application scientists during lecture and discussion sessions.

This 1-day class includes on-site training as prearranged by an Applied Biosystems applications scientist.

Available by request. Please call 650-554-3360 or email customertraining@lifetech.com for more information or to register.

The course is designed for individuals who will be directly involved in generating libraries from RNA to run on a SOLiD™ System.

Ownership of a SOLiD™ System (or affiliation with a laboratory that has purchased a system) and prior knowledge and basic experience of molecular biology and laboratory techniques including polymerase chain reaction (PCR) and pipetting. Past attendance of the SOLiD™ System Training Course is recommended.

This is a 3-day intensive course that gives participants a strong practical and theoretical knowledge of how to build SAGE libraries using the SOLiD™ Total RNA-Seq Kit, with Tips & Tricks to QC and troubleshoot libraries. (Please note, we will NOT be able to use customer samples for library construction during this course.)

Tuition at customer site includes course materials. Available by request. Please call 650-554-3360 or email customertraining@lifetech.com for more information or to register.

The course is designed for individuals who will be directly involved in generating libraries from RNA to run on a SOLiD™ System. Prerequisites: Ownership of a SOLiD™ System (or affiliation with a laboratory that has purchased a system) and prior knowledge and basic experience of molecular biology and laboratory techniques including polymerase chain reaction (PCR) and pipetting. Past attendance of the SOLiD™ System Training Course is recommended.

This is a 3-day intensive course that gives participants a strong practical and theoretical knowledge of how to build whole transcriptome and small RNA libraries using the SOLiD™ Total RNA-Seq Kit, with tips & tricks to QC and troubleshoot libraries. (Please note, we will NOT be able to use customer samples for library construction during this course.)

Tuition at customer site includes course materials. Available by request. Please call 650-554-3360 or email customertraining@lifetech.com for more information or to register.

Designed for individuals generating targeted resequencing libraries with the Agilent SureSelect&trade** Target Enrichment System to run on a SOLiD&trade System.

Ownership of a SOLiD&trade System (or belonging to a laboratory that has purchased a system) is a prerequisite for registration. Ideally, individuals have already participated in the SOLiD™ System Training Course, although this is not mandatory. This class assumes attendees will have prior knowledge of biological systems and basic experience with laboratory techniques, such as pipetting and PCR.

An approximately 3-day intensive course providing strong practical and theoretical knowledge of construction of targeted resequencing libraries. Participants will perform targeted enrichment of selected regions of the genome enrichment (with the Agilent SureSelect&trade** Target Enrichment System) and construct the resulting enriched library using genomic DNA provided by the training lab. (Please note, we will NOT be able to use customer samples for library construction during this course.) Theory behind enrichment and library construction and an in-depth evaluation of each critical stage of the workflow is reviewed. Participants will also be provided with best practices on library QC methods, laboratory preparation, and equipment calibration before embarking on the creation of their own libraries.

Tuition at customer site includes course materials. Available by request. Please call 650-554-3360 or email customertraining@lifetech.com for more information or to register.

**SureSelect® is a trademark of Agilent Technologies, Inc.

Designed for individuals preparing Methylation or ChIP-Seq libraries to run on the SOLiD™ System.

Ownership of a SOLiD™ System (or belonging to a laboratory that has purchased a system) is a prerequisite for registration. Ideally, individuals have already participated in the SOLiD™ System Training Course, although this is not mandatory. This class assumes attendees will have prior knowledge of biological systems and basic experience with laboratory techniques, such as pipetting and PCR.

A 5-day intensive course providing a strong practical and theoretical knowledge of construction of Methylation enriched libraries and ChIP-Seq libraries using the MethylMiner™ and SOLiD™ ChIP-Seq Kits available through Life Technologies. (Please note, we will NOT be able to use customer samples for library construction during this course.)

Tuition at customer site includes course materials. Available by request. Please call 650-554-3360 or email customertraining@lifetech.com for more information or to register.