Human Identification

31xx and 310 classes are available and course content reflects the instrument chosen. Dates listed here are for 31xx classes only. Please contact us for custom scheduling for 310-specific classes.

P/N for 310 class at AB site: 4327715

Course lectures and laboratory exercises

• DNA quantification using the Quantifiler® Duo Kit
• Short Tandem Repeat overview
• PCR amplification using the AmpFℓSTR® Identifiler® and Yfiler® PCR Amplification Kits
• Set-up and operation of the 96-well GeneAmp® 9700 PCR System
• 7500 Real-Time PCR System set-up and operation
• 3130 Series Genetic Analyzer instrument set-up and operation
• Data analysis using GeneMapper® ID-X or GeneMapper® ID software v3.2.1
• Data interpretation and troubleshooting
• Instrument preventative maintenance

Course Content: During this 4-day interactive lecture and laboratory course, students receive training on the Applied Biosystems Quantifiler® Duo Kit and the AmpFℓSTR® Identifiler® and Yfiler® PCR Amplification Kits. The curriculum emphasizes the importance of combining Applied Biosystems validated Human Identification reagents with instrumentation and software for optimal performance. Attendees utilize the most advanced solutions available for Human Identification applications, including DNA quantification using the Quantifiler® Duo kit and the 7500 Real-Time PCR System, amplification of single source and mixed samples using the AmpFℓSTR® Identifiler® and Yfiler® kits, and genotyping on the 3130 Genetic Analyzer. In addition, hands-on training with the GeneMapper® ID-X or ID Analysis Software enables analysts to gain a better understanding of how to configure the software and utilize it to reduce the time needed to analyze AmpFℓSTR® amplification kit data.

This hands-on laboratory work, coupled with the interactive lectures, will help students better understand when and how to use the tools available for human identification applications.

Training course fees include course materials, a four-night stay at a designated local hotel near the training facility, continental breakfast, lunch, snacks for each day, and a group dinner with the trainers. An on-line knowledge assessment will be available after the class conferring a certificate of completion.

An instrument serial number is required for registration. Applied Biosystems reserves the right to cancel courses three weeks prior to each class. Please take this into consideration before making travel arrangements.

For any questions regarding this course please contact the Training Manager at: hid_university@appliedbiosystems.com

Course Content: This 2-day course presents in-depth training on the operation and maintenance of the 3500 Genetic Analyzer for forensics and paternity laboratories. Interactive lectures and hands-on exercises with the 3500 will provide detailed instruction on the new Data Collection interface, calibrations, setting up a plate, within Data Collection data pre-review, generating reports, and data normalization. An introduction to GeneMapper® ID-X v. 1.2 software data analysis will cover the basics of data analysis, the Analysis Summary page, the profile comparison tool, setting up for data normalization and reports.

This hands-on laboratory work, coupled with the interactive lectures, will help students better understand when and how to use the tools available for human identification applications.

Training course fees include course materials, a two-night stay at a designated local hotel near the training facility, continental breakfast, lunch, snacks for each day, and a group dinner with the trainers.

An instrument serial number is required for registration. Applied Biosystems reserves the right to cancel courses three weeks prior to each class. Please take this into consideration before making travel arrangements.

For any questions regarding this course please contact the Training Manager at: hid_university@appliedbiosystems.com

Course Content: This 2-day course provides in-depth training on DNA quantification for HID using TaqMan® Real-Time PCR technology. Real-time PCR theory will be discussed and hands-on laboratory exercises will utilize the Quantifiler® Duo DNA Quantification Kit on the 7500 Real-Time PCR System. Interpretation of results will be discussed and strategies for the determination of the most appropriate downstream STR amplification kit will be presented. An online knowledge assessment will be available after the class conferring a certificate of completion.

An instrument serial number is required for registration. Applied Biosystems reserves the right to cancel courses three weeks prior to each class. Please take this into consideration before making travel arrangements.

Course Content: This 1-day course provides in-depth training on DNA extraction for HID using the PrepFiler Forensic DNA Extraction Kit. An overview of the chemistry will be discussed and hands-on laboratory exercises including extraction and quantification will demonstrate how to utilize the PrepFiler Kit to extract DNA from forensic samples for optimal downstream STR profiling results.

An instrument serial number is required for registration. Applied Biosystems reserves the right to cancel courses three weeks prior to each class. Please take this into consideration before making travel arrangements.

Course Content: This 3-day class provides in-depth instruction on the operation of the HID EVOlution� System, an integrated, HID-validated automation system that combines the Tecan Freedom EVO® 150 automated liquid-handling workstation with Applied Biosystems instruments and reagents using application specific software and protocols. Topics covered include the theory of operation, running validated scripts for extraction, quantification, normalization and STR amplification, as well as troubleshooting. Participants will engage in hands-on workflows on the Tecan Freedom EVO® 150 robotic platform.

Training course fees include course materials, a three-night stay at a designated local hotel near the training facility, continental breakfast, lunch, snacks for each day, and a group dinner with the trainers. Participants receive course training materials and a Certificate of Completion at the conclusion of the course.

An instrument serial number is required for registration. Applied Biosystems reserves the right to cancel courses three weeks prior to each class. Please take this into consideration before making travel arrangements.

These training sessions will be set up on an as-needed basis. If you are interested in scheduling a time, please contact your local Applied Biosystems� Sales Representative or the Training Manager at HID_University@appliedbiosystems.com.

Course Content: This 3-day training provides in-depth instruction on the advanced features of GeneMapper® ID-X Software, including installation in a multi-user database environment, optimization for use as an Expert System, analysis requirement check, allelic ladder quality assessment, analysis summary tool, comprehensive quality value system, manual review tools, chain-of-custody and other security/audit features, quality control features, the report manager and the mixture analysis tool. Working knowledge of GeneMapper® ID v3.2.1 or GeneMapper® ID-X is required to participate. An online knowledge assessment will be available after the class conferring a certificate of completion.

Training course fees include course materials, a three-night stay at a designated local hotel near the training facility, continental breakfast, lunch, snacks for each day, and a group dinner with the trainers.

An instrument serial number is required for registration. Applied Biosystems reserves the right to cancel courses three weeks prior to each class. Please take this into consideration before making travel arrangements.

Course Content: This 4-day class provides in-depth training on methods to maximize the recovery of interpretable STR profiles from challenging samples, including difficult mixtures and degraded and/or inhibited samples. Strategies for handling difficult samples will be discussed, while hands-on laboratory exercises will utilize the most advanced HID solutions available including DNA quantification with the Quantifiler® Duo kit on the 7500 Real-Time PCR system; STR amplification with the AmpFℓSTR® Identifiler®, Yfiler® and MiniFiler� PCR amplification kits; capillary electrophoresis on the 3130 Genetic Analyzer; and analysis of single source and mixed source samples using GeneMapper® ID-X Software. An on-line knowledge assessment will be available after the class conferring a certificate of completion.

Class ends at 12:00pm on the last day.

Training course fees include course materials, a four-night stay at a designated local hotel near the training facility, continental breakfast, lunch, snacks for each day, and a group dinner with the trainers.

An instrument serial number is required for registration. Applied Biosystems reserves the right to cancel courses three weeks prior to each class. Please take this into consideration before making travel arrangements.

For any questions regarding this course please contact the Training Manager at: hid_university@appliedbiosystems.com

Course Content: This 3-day course taught by an Applied Biosystems Field Applications Specialist (FAS) and a Field Service Engineer (FSE) provides participants with extensive specialized knowledge for troubleshooting Applied Biosystems HID-validated instruments, reagents and software. This includes capillary electrophoresis instruments, real-time PCR instruments, Quantifiler® and AmpFℓ STR® assays, and GeneMapper® ID or ID-X software. With a focus on maximizing data quality throughout the forensic workflow, this in-depth course arms the participant with the highest level of troubleshooting skills available. An on-line knowledge assessment will be available after the class conferring a certificate of completion.

Training course fees include course materials, a three-night stay at a designated local hotel near the training facility, continental breakfast, lunch, snacks for each day, and a group dinner with the trainers.

An instrument serial number is required for registration. Applied Biosystems reserves the right to cancel courses three weeks prior to each class. Please take this into consideration before making travel arrangements.

This 1-day in-lab training is provided by an Applied Biosystems Field Service Engineer (FSE) or Field Applications Specialist (FAS) after instrument installation. Topics covered include 310 or 31XX series Genetic Analyzer overview and maintenance; Data Collection and computer maintenance; overview of workflow; basics of PCR, STRs and STR amplification kits; basics of capillary electrophoresis; introduction to GeneMapper® ID-X or ID Software; and basic troubleshooting. An online knowledge assessment will be available after the class conferring a certificate of completion.

This 1-day in-lab training is provided by an Applied Biosystems Field Service Engineer (FSE) or Field Applications Specialist (FAS) after instrument installation. Topics covered include the basics of real-time PCR for quantification of DNA, instrument operation and maintenance, assay workflow, analysis of real-time quantification data and basic troubleshooting. An online knowledge assessment will be available after the class conferring a certificate of completion.

31xx and 310 classes are available and course content reflects the instrument chosen. Dates listed here are for 31xx classes only. Please contact us for custom scheduling for 310-specific classes.

P/N for 310 class at customer site: 4327716

Pre-requisites: 100, 110 and 120 classes or completion of on-line knowledge assessments.

Course lectures and laboratory exercises

• DNA quantification using the Quantifiler® Duo Kit
• Short Tandem Repeat overview
• PCR amplification using the AmpFℓSTR® Identifiler® and Yfiler® PCR Amplification Kits
• Set-up and operation of the 96-well GeneAmp® 9700 PCR System
• 7500 Real-Time PCR System set-up and operation
• 3130 Series Genetic Analyzer instrument set-up and operation
• Data analysis using GeneMapper® ID-X or GeneMapper® ID software v3.2.1
• Data interpretation and troubleshooting
• Instrument preventative maintenance

Course Content: During this 4-day interactive lecture and laboratory course, students receive training on the Applied Biosystems Quantifiler® Duo Kit and the AmpFℓSTR® Identifiler® and Yfiler® PCR Amplification Kits. The curriculum emphasizes the importance of combining Applied Biosystems validated Human Identification reagents with instrumentation and software for optimal performance. Attendees utilize the most advanced solutions available for Human Identification applications, including DNA quantification using the Quantifiler® Duo kit and the 7500 Real-Time PCR System, amplification of single source and mixed samples using the AmpFℓSTR® Identifiler® and Yfiler® kits, and genotyping on the 3130 Series Genetic Analyzer. In addition, hands-on training with the GeneMapper® ID Analysis Software enables analysts to gain a better understanding of how to configure the software and utilize it to reduce the time needed to analyze AmpFℓSTR® amplification kit data.

This hands-on laboratory work, coupled with the interactive lectures, will help students better understand when and how to use the tools available for human identification applications. An on-line knowledge assessment will be available after the class conferring a certificate of completion.

An instrument serial number and a minimum of five registrants are required. Applied Biosystems reserves the right to cancel courses three weeks prior to each class.

Pre-requisites: 100, 110 and 120 classes or completion of on-line knowledge assessments.

Course Content: This 2-day course provides in-depth training on DNA quantification for HID using TaqMan® Real-Time PCR technology. Real-time PCR theory will be discussed and hands-on laboratory exercises will utilize the Quantifiler® Duo DNA Quantification Kit on the 7500 Real-Time PCR System. Interpretation of results will be discussed and strategies for the determination of the most appropriate downstream STR amplification kit will be presented. An online knowledge assessment will be available after the class conferring a certificate of completion.

Pre-requisites: 100, 110 and 120 classes or completion of on-line knowledge assessments. 200 class recommended or completion of on-line assessment for 200 class.

Pre-requisites: 100, 110 and 120 classes or completion of on-line knowledge assessments. 200 class recommended or completion of on-line assessment for 200 class.

Course Content: This 1-day course provides an overview of STR data analysis using GeneMapper® ID-X Software. The basic workflow will be outlined using example data to demonstrate how the software tools help to streamline and improve the forensic data analysis process. Basic troubleshooting is also covered. Instructor-led, hands-on exercises facilitate learning and enhance retention of material.

Pre-requisites: 100, 110 and 120 classes or completion of on-line knowledge assessments. 200 class recommended or completion of on-line assessment for 200 class.

Course Content: This 1-day course provides in-depth training on DNA extraction for HID using the PrepFiler™ Forensic DNA Extraction Kit. An overview of the chemistry will be discussed and hands-on laboratory exercises including extraction and quantification will demonstrate how to utilize the PrepFiler™ Kit to extract DNA from forensic samples for optimal downstream STR profiling results.

Pre-requisites: 100, 110 and 120 classes or completion of on-line knowledge assessments. 200 class recommended or completion of on-line assessment for 200 class.

Course Content: This 3-day class provides in-depth instruction on the operation of the HID EVOlution System, an integrated, HID-validated automation system that combines the Tecan Freedom EVO® 150 automated liquid-handling workstation with Applied Biosystems instruments and reagents using application specific software and protocols. Topics covered include the theory of operation, running validated scripts for extraction, quantification, normalization and STR amplification, as well as troubleshooting. Participants will engage in hands-on workflows on the Tecan Freedom EVO® 150 robotic platform.

Pre-requisites: 100, 110 and 120 classes plus 230 or 240 or completion of on-line knowledge assessments. 200 class recommended.

Course Content: This 3-day training provides in-depth instruction on the advanced features of GeneMapper® ID-X Software, including installation in a multi-user database environment, optimization for use as an Expert System, analysis requirement check, allelic ladder quality assessment, analysis summary tool, comprehensive quality value system, manual review tools, chain-of-custody and other security/audit features, quality control features, the report manager and the mixture analysis tool. Working knowledge of GeneMapper® ID v3.2.1 or GeneMapper® ID-X is required to participate. An online knowledge assessment will be available after the class conferring a certificate of completion.

Pre-requisites: 100, 110, 120 and 200 classes or completion of on-line knowledge assessments. 240 or 300 class recommended.

Course Content: This 4-day class provides in-depth training on methods to maximize the recovery of interpretable STR profiles from challenging samples, including difficult mixtures and degraded and/or inhibited samples. Strategies for handling difficult samples will be discussed, while hands-on laboratory exercises will utilize the most advanced HID solutions available including DNA quantification with the Quantifiler® Duo kit on the 7500 Real-Time PCR system; STR amplification with the AmpFℓSTR® Identifiler®, Yfiler® and MiniFiler� PCR amplification kits; capillary electrophoresis on the 3130 Genetic Analyzer; and analysis of single source and mixed source samples using GeneMapper® ID-X Software. An on-line knowledge assessment will be available after the class conferring a certificate of completion.

Pre-requisites: 100, 110 and 120 classes or completion of on-line knowledge assessments. 200 class recommended.

Course Content: This 3-day course taught by an Applied Biosystems Field Applications Specialist (FAS) and a Field Service Engineer (FSE) provides participants with extensive specialized knowledge for troubleshooting Applied Biosystems HID-validated instruments, reagents and software. This includes capillary electrophoresis instruments, real-time PCR instruments, Quantifiler� and AmpFℓ STR® assays, and GeneMapper® ID or ID-X software. With a focus on maximizing data quality throughout the forensic workflow, this in-depth course arms the participant with the highest level of troubleshooting skills available. An on-line knowledge assessment will be available after the class conferring a certificate of completion.